Cytogenetic Analysis of Solid Tissue

Clinical info Although peripheral blood is the most easily obtainable tissue for chromosome analysis, it is sometimes necessary to study solid tissue.
Indications
  • Suspicion of chromosome mosaicism; Mosaicism may be suspected based on previous blood chromosomes or due to phenotypic features
  • Blood is not available on the patient, but solid tissue is available - this may be the case when studying products of conception and abortus tissue, or when solid tissue is obtained from surgical or post-mortem procedures
    • Specimen Requirements Skin Biopsy: Using sterile technique, obtain a 5 mm biopsy of tissue and place in a sterile tube containing transport media. If tissue culture media is not available, sterile solutions such as balanced salt solution may be used.
      Products of Conception: Products of conception should be placed in a sterile container for transport. The specimen must be kept moist - there is usually sufficient fluid present to ensure moisture. However, if it is necessary to add a sterile solution, add tissue culture media or sterile saline, by aseptic technique.
      Abortus Tissue: Using sterile technique, obtain a 5 mm biopsy of unmacerated fetal tissue and place in tube containing transport media. If tissue culture media is not available, sterile solutions such as balanced salt solution may be used. If fetus is macerated, fetal tissue may not grow in culture; however, placental tissue will often be viable beyond the time that fetal tissue can be successfully cultured. The preferred placental tissues are fetal membranes or chorionic villi. If fetal samples are obtained at autopsy, lung, gonad or thymus are preferred for chromosome study.

      **Do not allow the specimen (of any tissue type) to come into contact with formalin or other fixatives as fixed tissue will not grow.
      Transport For all tissue types, specimen should be kept at room temperature if it will be transported immediately. If specimen is not being immediately transported to the laboratory, it may be refrigerated; do not freeze. Specimen should be sent by courier or overnight mail to arrive at the laboratory within 24 hours.
      Turnaround time 6 weeks
      Cell culture can take 1-4 weeks and may lengthen turnaround time.
      CPT Codes 88233, 88262, 88291
      Cost $704
      Contact For further information contact Kellie King, MS , Lab Counselor or Barb DuPont,PhD , Cytogenetics Laboratory Director at 1-800-473-9411

      Cytogenetics Lab

      The Cytogenetics Lab provides high quality analysis for the detection of both constitutional chromosomal aberrations as well as abnormalities related to hematological malignancies

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