Cytogenetic Analysis of Solid Tissue
|Clinical info||Although peripheral blood is the most easily obtainable tissue for chromosome analysis, it is sometimes necessary to study solid tissue.|
|Specimen Requirements||Skin Biopsy: Using sterile technique, obtain a 5 mm biopsy of tissue and place in a sterile tube containing transport media. If tissue culture media is not available, sterile solutions such as balanced salt solution may be used.
Products of Conception: Products of conception should be placed in a sterile container for transport. The specimen must be kept moist - there is usually sufficient fluid present to ensure moisture. However, if it is necessary to add a sterile solution, add tissue culture media or sterile saline, by aseptic technique.
Abortus Tissue: Using sterile technique, obtain a 5 mm biopsy of unmacerated fetal tissue and place in tube containing transport media. If tissue culture media is not available, sterile solutions such as balanced salt solution may be used. If fetus is macerated, fetal tissue may not grow in culture; however, placental tissue will often be viable beyond the time that fetal tissue can be successfully cultured. The preferred placental tissues are fetal membranes or chorionic villi. If fetal samples are obtained at autopsy, lung, gonad or thymus are preferred for chromosome study.
**Do not allow the specimen (of any tissue type) to come into contact with formalin or other fixatives as fixed tissue will not grow.
|Transport||For all tissue types, specimen should be kept at room temperature if it will be transported immediately. If specimen is not being immediately transported to the laboratory, it may be refrigerated; do not freeze. Specimen should be sent by courier or overnight mail to arrive at the laboratory within 24 hours.|
|Turnaround time||6 weeks
Cell culture can take 1-4 weeks and may lengthen turnaround time.
|CPT Codes||88233, 88262, 88291|
The Cytogenetics Lab provides high quality analysis for the detection of both constitutional chromosomal aberrations as well as abnormalities related to hematological malignancies